3D Ex vivo tissue platforms to investigate the early phases of influenza a virus- and SARS-CoV-2-induced respiratory diseasesArticle Published on 2022-12-012022-11-15 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 신약개발, 진단, 치료제, [키워드] animal model animal models Antiviral Cell Cell culture comparable consequence Drug repurposing drug target early phase Ex vivo ex vivo infection Ex vivo infection model Health Host human lung IAV in vitro Infection Inflammatory cytokine Influenza influenza A virus Influenza virus Innate immunity innate immunity. interferon interferon response Intervention introduced lung tissue morbidity and mortality murine outbreak pathophysiological pharmacological platform Respiratory disease respiratory viruses responses SARS-CoV-2 therapy tissue Treatment viral infection viral life cycle viral replication virus virus while [DOI] 10.1080/22221751.2022.2117101 PMC 바로가기
Pulmonary delivery of favipiravir inhalation solution for COVID-19 treatment: in vitro characterization, stability, in vitro cytotoxicity, and antiviral activity using real time cell analysisArticle Published on 2022-12-012022-11-15 Journal: Drug Delivery [Category] COVID19(2023년), SARS, 치료제, [키워드] addition administration Analysis Antiviral antiviral activity Calu-3 Cell characterized Concentration COVID-19 cytotoxicity Favipiravir in vitro indicated Influenza virus Inhaled inhaled formulation inhibitor investigated lung lung epithelial cell lung tissue Lungs media Patient patients with COVID-19 PBS phosphate buffer profile pulmonary RdRP real time required respiratory. RNA-dependent RNA polymerase SARS-CoV-2 stability target Toxicity treat Treatment VERO-E6 viability were infected [DOI] 10.1080/10717544.2022.2118398 PMC 바로가기
Induction of pulmonary HLA-G expression by SARS-CoV-2 infectionArticle Published on 2022-11-052022-11-15 Journal: Cellular and Molecular Life Sciences [Category] SARS, 치료기술, 치료제, [키워드] accompanied Antigen Autopsy biopsies CD8 Cell changes in correlated distribution epithelial cell Evidence expressed expression expression level Frequency heart muscle HLA-G Hyperactivation Immune cell Immune cell infiltration immune cells Immune escape immune response induction information kidney leukocyte lung lung alveolar lung epithelia lung epithelial cell lung tissue Macrophage marker microRNA microRNA. miRNA miRNA expression modulating organs other organ patients protein expression SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 infections SARS-CoV-2 nucleocapsid SARS-CoV-2- SARS-CoV-2-infected patient specimen Spike protein surgical T cell responses T cells therapeutic target tissue tissues Treatment Tumor virus-infected cells while [DOI] 10.1007/s00018-022-04592-9 PMC 바로가기
Transcriptional regulation and small compound targeting of ACE2 in lung epithelial cellsArticle Published on 2022-11-012022-11-15 Journal: Acta pharmacologica Sinica [Category] COVID19(2023년), SARS, 진단, [키워드] 16HBE 16HBE cell 16HBE cells 6-O-angeloylplenolin ACE2 ACE2 expression addition administration Cell Centipeda Centipeda minima chemokine Chinese Compound conducted COVID-19 CX3CL1 CXCL13 dataset demonstrated downregulated entry of SARS-CoV-2 enzyme evaluated expression gene encoding HLF IL-6 inhibited inhibitor Interaction lung epithelial cell Lung epithelial cells lung tissue lung tissues mice modulate parental pathogen phosphorylated positively correlated promoter PROTECT Protein pseudovirus receptor reduced regulate Regulation S protein SARS-CoV-2 SARS-COV-2 infection screened STAT3 STAT3. stimulated suppressed target cells tested TFS Transcription transcription factor transfected with [DOI] 10.1038/s41401-022-00906-6 PMC 바로가기
B.1.351 SARS-CoV-2 Variant Exhibits Higher Virulence but Less Viral Shedding than That of the Ancestral Strain in Young Nonhuman PrimatesArticle Published on 2022-10-262022-11-16 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, 유전자 메커니즘, [키워드] addition Alpha animal model association B.1.351 Beta caused changes in Characteristics Chinese circulating Clinical signs contribute determine distribution drugs early stage Efficacy and safety elevated exhibited IFN-γ IL-17 Infection Inflammatory Inflammatory cytokine interferon gamma interleukin 17 investigated less Lower respiratory tract lung tissue mechanisms mutated Necrosis NHP NHPs nonhuman primate pathogenesis of SARS-CoV-2 pathogenic pathogenicity pathological changes primate provide pulmonary inflammation reduced replicated Research rhesus macaque SARS-CoV-2 SARS-CoV-2 strain SARS-CoV-2 vaccine SARS-CoV-2 variant SARS-CoV-2 variants severe lung injury shedding spike-specific T-cell response strain Strains tissue TNF-α Transmission upper respiratory tract Vaccine variant variants of concern viral distribution viral shedding virulence virulence. virulent VoC VOCs were infected [DOI] 10.1128/spectrum.02263-22 PMC 바로가기
Inhaled aviptadil for the possible treatment of COVID-19 in patients at high risk for ARDS: study protocol for a randomized, placebo-controlled, and multicenter trialArticle Published on 2022-09-202022-11-16 Journal: Trials [Category] SARS, 변종, [키워드] acute lung injury acute respiratory distress addition adverse effects antibody applied ARDS Aviptadil baseline Clinical improvement clinical status Concentration conducted COVID-19 COVID-19 in patient COVID-19 patient defined double-blinded Efficacy and safety Europe exclusion criteria FIVE hemodynamic instability high risk homeostasis hospital discharge Hospitalization immunomodulating IMPROVE indicate Inhaled intensive care intestinal intravenously lung lung tissue management mechanical ventilation metabolism Mortality multicenter Multicenter trial pandemic Pathways Patient patients hospitalized peptide placebo-controlled Prevent primary endpoint pro-inflammatory Prognosis Protein Public Hospital Randomized randomized trial Randomly reduction Regulation respiratory complication Risk factors SARS-CoV-2 severe disease course shown standard care stratified Study protocol suggested syndrome the SARS-CoV-2 therapeutic agent Therapies therapy Treatment unique vaccination Vaccine variants of SARS-CoV-2 Vasoactive VIP VIP. with COVID-19 World Health Organization [DOI] 10.1186/s13063-022-06723-w PMC 바로가기
PD-1 and ICOS counter-regulate tissue resident regulatory T cell development and IL-10 production during fluArticle Published on 2022-09-082022-11-15 Journal: Frontiers in Immunology [Category] 바이오마커, [키워드] accumulated CD4 Cell characterized co-stimulatory receptor COVID Critical Express expressed flu fluorescent FOXP3 GFP heterogenous human population iCoS IL-10 Immune homeostasis immunopathology in vivo Infection infections Influenza influenza infection influenza infections labeling lung tissue memory T cells molecular feature PD-1 proinflammatory promote Regulatory Regulatory T cell repeated reporter respiratory infectious disease response reveal secondary infections shown Signaling signaling pathway suppress T cell T cell response T cells tissue tissue repair tissue resident T cells. transcription factor Treg Treg cell Treg cells Tuberculosis [DOI] 10.3389/fimmu.2022.984476 PMC 바로가기
Cleavage-Responsive Biofactory T Cells Suppress Infectious Diseases-Associated HypercytokinemiaArticle Published on 2022-09-012022-11-15 Journal: Advanced Science [Category] 바이오마커, [키워드] activate administration Alpha Blood chimeric cleavage cleavage site coronavirus disease COVID-19 digestion Diseases domain drug endothelial engineered blood vessel engineered T cell enzyme EPCR Express expressing hypercytokinemia immune cells in vivo induce Infectious disease Infectious disease. lentiviral system lung tissue multiple organ failure Necrosis overcome peptide Prevent Protein receptor Release Sepsis suppress T cell therapeutic thrombin receptor TRAP Treatment Vascular damage vessel [DOI] 10.1002/advs.202201883 PMC 바로가기
Novel application of [18F]DPA714 for visualizing the pulmonary inflammation process of SARS-CoV-2-infection in rhesus monkeys (Macaca mulatta)Article Published on 2022-09-012022-11-16 Journal: Nuclear medicine and biology [Category] COVID19(2023년), SARS, 치료기술, [키워드] Activation added BAL Blood Bronchoalveolar lavage Chest COVID-19 dendritic cell determine diagnostic increased expression Infection infection process Inflammation initial injection lesions lung lung tissue Lymph node marker nonhuman primates novel performed pet PET-CT positive post infection principal pulmonary pulmonary inflammation pulmonary lesion pulmonary lesions Rhesus monkey Rhesus monkeys SARS-CoV-2 Seven Swab swabs timepoint TSPO TSPO. viral respiratory infections [DOI] 10.1016/j.nucmedbio.2022.05.002 PMC 바로가기
Replication of SARS-CoV-2 Omicron BA.2 variant in ex vivo cultures of the human upper and lower respiratory tractArticle Published on 2022-09-012022-11-16 Journal: EBioMedicine [Category] SARS, 변종, [키워드] airway antibody bronchial Bronchial tissue characterized China clinical manifestation Committee comparable Culture Delta delta variant disease severity Ex vivo explain explained Hong Kong human lung tissue immune evasion Infectious disease Lower respiratory tract lung lung tissue nasal nasal tissue observation omicron Omicron BA.2 parenchymal pathogenicity Patient Region replaced replicated Replication respiratory tissue SARS-CoV-2 strain Strains supported tested tissue Transmissibility Transmission. university variant virus viruses wild-type wild-type virus [DOI] 10.1016/j.ebiom.2022.104232 PMC 바로가기